CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

72007

$1,070 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-1 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against CD28:B7-1[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

CD80, B7.1, activity assay kit, Strep HRP, streptavidin

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified CD28, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
71114	B7-1, Biotin-labeled	2 x 5 µg	-80°C	Avoid multiple freeze/ thaw cycles
71113	CD28	10 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
	HRP chemiluminescent substrate A (transparent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
79699	White 96-well microplate	1	+4°C

UniProt #

P10747

Background

The activation of naïve T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. CD28:B7-1 interaction is an important drug target for the regulation of T cells involved in autoimmunity, inflammation, tumor recognition, and immune tolerance.

References

1. Keir, M.E., et al. Immunol. Rev. 2005, 204: 128-143.
2. Haas, C., et al. Int. J. Cancer. 2006, 1;118: 658-667.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

This kit is useful for screening for inhibitors of B7-1 binding to CD28.

Shipping Temperature

-80°C (dry ice)

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cell Surface Receptor/Single-pass Transmembrane Protein.

Regulatory Status

Research Use Only

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CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit

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