CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit

Catalog #: 72007
Size: 96 reactions
$1,040
Background:
The activation of naïve T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. CD28:B7-1 interaction is an important drug target for the regulation of T cells involved in autoimmunity, inflammation, tumor recognition, and immune tolerance.
Description:

The CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-1 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

UniProt
P10747
Synonym(s):
CD80, B7.1
Supplied As:
This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified CD28, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
This kit is useful for screening for inhibitors of B7-1 binding to CD28.
Reference(s):

1. Keir, M.E., et al. Immunol. Rev. 2005, 204: 128-143.
2. Haas, C., et al. Int. J. Cancer. 2006, 1;118: 658-667.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Cell Surface Receptor/Single-pass Transmembrane Protein.
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

72007.pdf