CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

79695

$1,070 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD27:CD70 signaling. This kit comes in a convenient 96- well format, with biotin-labeled CD70, purified CD27, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD70 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD27 is coated on a 96-well plate. Next, CD70 is incubated with CD27 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against CD27:CD70[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

S152, LPFS2, CD27LG, TNFSF7

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format

Components

UniProt #

P26842

Background

The tumor necrosis factor (TNF) receptor family member CD27 (TNFRSF7) and its TNF-like ligand CD70 (CD27L, TNFSF7) are a key costimulatory immune checkpoint signal for lymphocyte activation. Among other effects, this interaction promotes the differentiation of T and B cells and participates in immunoglobulin synthesis. CD27:CD70 interaction has been demonstrated to have important roles in autoimmunity, inflammation, infection and cancer, and antibodies targeting either CD27 or CD70 are in clinical trials for oncology.

References

Granger, S.W., et al. Cytokine Growth Factor Rev. 2003, 14(3-4): 289-296

Storage/Stability

Up to 6 months from date of receipt, when stored as recommended.

Instructions for Use

See assay protocol.

Shipping Temperature

-80°C

Warnings

Avoid multiple freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit

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