CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit

Catalog #
72025
$1,130 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. This kit comes in a convenient 96-well format, with biotin-labeled CD137, purified CD137L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. This assay takes advantage of the highly sensitive detection of biotin-labeled CD137 by streptavidin-HRP. First, CD137L is coated on a 96-well plate. Next, CD137-biotin is incubated with CD137L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Synonyms
4-1BB, Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, ILA, 4-1BB ligand, 4-1BBL, tumor necrosis factor ligand superfamily member 9, TNFSF9, activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Supplied As
This kit comes in a convenient 96-well format, with biotin-labeled CD137, purified CD137L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied
  • PBS (Phosphate buffered saline)
  • Luminometer or microplate reader capable of reading chemiluminescence
  • Rotating or rocker platform
Format
Catalog
Number 

Component

Amount

Storage
71171 CD137 (4-1BB), His-tagged, Biotin-labeled 2 µg -80 °C
71189 CD137L (4-1BB ligand), His tagged 10 µg -80 °C
79742 Streptavidin-HRP  10 µl  +4°C 
79311 3x Immuno Buffer 1 50 ml -20 °C
79728 Blocking Buffer 2 50 ml +4 °C
79670  ELISA ECL substrate A
(transparent bottle)
6 ml Room Temp.
ELISA ECL substrate B
(brown bottle)
6 ml Room Temp.
79699 96-well white microplate 1 Room Temp.
UniProt #
CD137: Q07011; CD137L: P41273
Background

CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4-1BBL, results in increased s T-cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases. 

References

1. Drenkard, D., et al., FASEB J. 2007; 21: 456-463
2. Kwon, B., et al., Immune Netw. 2009; 9(3): 84-89.