CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit

Catalog #: 72025
Size: 96 reactions
$995
Background:
CD137, also known as 4-1BB, is a prototype co-stimulatory molecule expressed on T cells. CD137 interaction with its cognate ligand, CD137L, also known as 4- 1BBL, results in increased s T-cell activation and proliferation. Accumulating evidence shows a role for CD137:CD137L signaling in inflammation, suggesting that inhibition of this pathway may provide a therapeutic avenue to treat autoimmune and inflammatory diseases.
Description:

The CD137:CD137L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD137:CD137L interaction. The key to this kit is the high sensitivity of detection of biotin-labeled CD137 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD137L is coated on a 96-well plate. Next, CD137-biotin is incubated with CD137L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Synonym(s):
4-1BB, Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, ILA, 4-1BB ligand, 4-1BBL, tumor necrosis factor ligand superfamily member 9, TNFSF9, activity assay kit, Strep HRP, streptavidin
Supplied As:
This kit comes in a convenient 96-well format, with biotin-labeled CD137, purified CD137L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
This kit is useful for screening for inhibitors of CD137 binding to CD137L.
Reference(s):
1. Drenkard, D., et al., FASEB J. 2007; 21: 456-463
2. Kwon, B., et al., Immune Netw. 2009; 9(3): 84-89.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy/Cell Surface Receptor
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

72025.pdf