BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

72008

$1,070 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The binding of BTLA to the HVEM receptor (herpesvirus entry mediator, also known as TNFRSF14) triggers cell signaling pathways involved in the negative regulation of T cells. BTLA:HVEM interaction plays a key role in the regulation of inflammatory, autoimmune, and antitumor responses, and is an important target for drug discovery. The BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BTLA:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BTLA is coated on a 96-well plate. Next, HVEM is incubated with BTLA on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against BTLA:HVEM[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

This product has been cited 4 times.

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Synonyms

CD272, CD270, TNFRSF14, ATAR, HVEA, LIGHTR, TR2 , activity assay kit, Strep HRP, streptavidin

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Product Data Gallery

Inhibition of BTLA:HVEM interaction by BTLA antagonist antibody

Product Info

Storage and Usage

Citations4

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled HVEM, purified BTLA, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
71141	BTLA	10 µg	-80°C	Avoid freeze/ thaw cycles!
71143	HVEM, Biotin-labeled	5 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
79311	3X Immuno Buffer	50 ml	-20°C
79728	Blocking buffer 2	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp
79699	White 96-well microplate	1	+4°C

References

1. Derre L., et al. J. Clin. Invest. 2010, 120(1): 157-167.
2. Steinberg, M.W., et al. J. Exp.Med. 2008, 205(6): 1463-1476.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

This kit is useful for screening inhibitors of HVEM binding to BTLA

Shipping Temperature

-80°C (dry ice)

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cell Surface Receptor/Single-pass Transmembrane Protein

Regulatory Status

Research Use Only

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BTLA:HVEM[Biotinylated] Inhibitor Screening Assay Kit

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