Anti-PD-1 Neutralizing Antibody, PE-labeled

Catalog #: 71290-1
Size: 50 ug
MW: 150 kDa
R-Phycoerythrin-labeled anti-PD-1 neutralizing recombinant murine/human chimeric antibody recognizing the PD-L1/PD-L2 binding region of human PD-1. This antibody does not cross-react with mouse PD-1, but does cross-react with monkey (M. fascicularis) PD-1. It has not been tested with other species.
Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L
Assay Conditions:
For flow cytometry, we recommend incubating cells on ice for 30 minutes with 1 – 20 µg/ml of RPE anti-human PD-1 antibody.
5.14 mg/ml
Protein A affinity chromatography from HEK293 supernatants.
Storage / Stability:
Stable for at least 12 months at -80°C.
Useful for labeling cells expressing PD-1 for flow cytometry and immunofluorescence microscopy.

1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.
2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.

Application References:

1. PD-1 blockade restores impaired function of ex vivo expanded CD8+ T cells and enhances apoptosis in mismatch repair deficient EpCAM+PD-L1+ cancer cells (2017)

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