Human Interleukin-33 Recombinant

Catalog #

90191-A

$130 *

Size: 2 µg

Qty

Bulk/Custom

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Description

Recombinant Interleukin-33 is a disulfide-linked monomer protein consisting of 160 amino acid residues, and migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-33 mature chain was expressed in E. coli.

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Synonyms

IL-33, interleukin 33, IL33, IL1RL1 ligand

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.5 (1 mg/ml).

18 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

112–270

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of murine D10S cells is ≤ 0.03 ng/ml.

Genbank #

O95760

UniProt #

O95760

Background

IL-33, a member of the IL-1 family, signals via a heterodimeric receptor complex consisting of ST2 and IL-1R accessory protein and triggers the activation of NF-kB and all three MAPKs (p38, ERK1/2, and JNK1/2) in mast cells. IL-33 is expressed in multiple tissues and by several cell types such as dermal fibroblasts and small airway epithelial and bronchial smooth muscle cells. IL-33 activated IL-1-like signaling responses in mast cells and enhanced IL-5 and IL-13 production from murine Th2-polarized splenocytes. Moreover, it was found that both human and murine mast cells produced a wide spectrum of cytokines and chemokines when stimulated in vitro with IL- 33. In addition, IL-33 enhanced IL-4-driven Th2 cell responses and acted as a selective chemoattractant for Th2 cell recruitment.

References

1. J. Immunol., Feb 2010, 184: 1526 - 1535.
2. J. Immunol., Dec 2009, 183: 7890 - 7897.
3. J. Immunol., Nov 2009, 183: 6469 - 6477.

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Human Interleukin-33 Recombinant

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