Human Interleukin-3 Recombinant

Catalog #

90188-A

$130 *

Size: 2 µg

Qty

Bulk/Custom

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Description

Recombinant IL-3 is a disulfide-linked monomeric protein consisting of 134 amino acid residues, and migrates as an approximately 15 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-3 mature chain was expressed in E. coli.

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Synonyms

IL-3, interleukin 3, Mast Cell Growth Factor, MCGF, IL3

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥97% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.

15 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

20–152

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of Human TF-1 cells was found less than 0.1 ng/ml.

Genbank #

P08700

UniProt #

P08700

Background

IL-3 is produced mainly by T- cells following cell activation by antigens and mitogens, but also by keratinocytes, NK-cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial- derived recombinant IL-3 shows that glycosylation is not required for the activity of IL-3. IL-3 sequences are evolutionarily not well conserved. Human and murine IL-3 show approximately 29% homology at the protein level while murine and rat IL-3 show approximately 54% homology. IL-3-alpha and IL-3- beta are two isoforms of rat IL-3. IL-3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells, and various myeloid leukemia cells. IL-3/IL-3 receptor complexes have a Kds of 10^-9 - 10^-10 M. Binding of IL-3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein.

References

1. Mol. Cell. Biol., Apr 2009, 29: 1682 - 1693.
2. J. Immunol., Jan 2009, 182: 1050 - 1060.
3. J. Immunol., Feb 2009, 182: 2432 - 2438.

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Human Interleukin-3 Recombinant

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