Mouse Interleukin-2

Catalog #: 90185-B
Size: 20 µg
$200
Species: Mouse
Host Species: E. coli
MW: 15 kDa
Genbank #: P04351
a.a: 22–169
Background:
IL-2 is produced mainly by T- cells expressing the surface antigen CD4 following cell activation by mitogens or allogen. Interleukin-2 (IL-2) has multiple, sometimes opposing, functions during an inflammatory response. It is a potent inducer of T-cell proliferation and T- helper 1 (Th1) and Th2 effector T-cell differentiation and provides T cells with a long-lasting competitive advantage resulting in the optimal survival and function of memory cells. In a regulatory role, IL-2 is important for the development, survival, and function of regulatory T cells, it enhances Fas-mediated activation-induced cell death, and it inhibits the development of inflammatory Th17 cells. Thus, in its dual and contrasting functions, IL-2 contributes to both the induction and the termination of inflammatory immune responses.
Description:
Recombinant IL-2 is a disulfide-linked monomeric protein consisting of 150 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding murine Interleukin-2 mature chain was expressed in E. coli.
UniProt
P04351
Synonym(s):
IL-2, Lymphokine, TCGF, T Cell Growth Factor, Aldesleukin, Interleukin-2, IL2, CD122
Purity:
≥95% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the dose dependent stimulation of murine CTLL-2 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
Formulation:
Lyophilized from 0.2 µm filtered PBS, pH 7.0.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Reference(s):

1. Shevach EM. Trends Immunol. 2012 Dec,33(12):626-32.
2. Dooms H, Abbas AK. Eur J Immunol. 2010 Jun,40(6):1538-40.

Application Reference(s):
1. Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse (2017)

Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
PTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENFISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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