Human Interleukin-13 Recombinant

Catalog #

90179-A

$130 *

Size: 2 µg

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Bulk/Custom

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Description

Recombinant IL-13 is a disulfide-linked monomer protein consisting of 115 amino acid residues,and migrates as an approximately 13 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-13 mature chain was expressed in E. coli.

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Synonyms

IL-13, IL13, ALRH, Allergic Rhinitis, BHR1, Bronchial Hyperresponsiveness-1 , Interleukin-13

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered concentrated (1 mg/ml) solution in PBS, pH 7.2.

13 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

19–132

Amino Acid Sequence

SP GPVPPSTALR ELIEELVNIT QNQKAPLCNG SMVWSINLTA GMYCAALESL INVSGCSAIE KTQRMLSGFC PHKVSAGQFS SLHVRDTKIE VAQFVKDLLL HLKKLFREGR FN

Biological Activity

The ED₅₀ was determined by the dose-dependent proliferation of TF-1 cells was ≤2.0 ng/ml, corresponding to a specific activity of ≥1 x 10⁶ units/mg.

Genbank #

P35225

UniProt #

P35225

Background

Mouse IL-13 is produced primarily by activated Th2 cells. Human IL-13 is expressed in activated T- helper cells (resembling Th0, Th1, Th2), and T-cells expressing CD8. The alpha chain of the IL1-3 receptor has weak binding activity for IL-13. High affinity receptors are formed when the receptor alpha chains of IL-13 and IL-4 receptors are coexpressed. The receptors for IL-13 and IL-4 share a common gamma subunit that is found also in the IL-2 receptor. Different IL-13 receptor structures have been shown to exist on various cell types and the IL-13 receptor may share more than one component with IL-4 receptor. IL-13 down-modulates macrophage activity, reducing the production of pro-inflammatory cytokines and chemokines in response to IFN-gamma or bacterial lypopolysaccharides. IL-13 enhances the production of the IL-1 receptor antagonist IL-1ra. IL-13 also decreases the production of nitric oxide by activated macrophages, leading to a decrease in parasiticidal activity. IL-13 induces differentiation of human monocytes, enhances survival time in culture, and also induces differentiation and proliferation and Isotype switching in B-cells.

References

1. J. Biol. Chem., Sep 2009, 284: 24289 - 24296.
2. J. Immunol., Aug 2009, 183: 1934 - 1939.

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Human Interleukin-13 Recombinant

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