Mouse Interleukin-10 Recombinant

Catalog #

90174-A

$130 *

Size: 2 µg

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Description

Recombinant Mouse IL-10 is a disulfide-linked monomer protein consisting of 161 amino acid residues, and migrates as an approximately 19 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding mouse Interleukin-10 mature chain was expressed in E. coli.

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Synonyms

IL-10, Interleukin 10, Cytokine Synthesis Inhibitory Factor, CSIF, TGIF, IL10

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

E. coli

Purity

≥95% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered PBS, pH 7.0.

19 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

19–178

Biological Activity

The ED₅₀ was determined by the dose-dependent (with IL-4) proliferation of murine MC/9 cells, and was found to be < 2 ng/ml, corresponding to a specific activity of > 5 x 10⁵ units/mg.

Genbank #

P18893

UniProt #

P18893

Background

IL-10 is produced by mouse Th2-cells following their stimulation by lectins. The main source for B-cell derived IL-10 in mice are Ly1 B-cells that express CD5 (Ly1) and CD11. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones after both antigen-specific and polyclonal activation, by B- cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. IL-10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin-10 shows 73% amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL-10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL-10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL-2 and TNF-beta in Th1 T-helper subpopulations of T-cells but not of Th2 T-helper cells. This activity is antagonized by IL-4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL-12 synthesis by accessory cells. In the human system, IL-10 is produced by, and down-regulates the function of Th1 and Th2 cells.

References

1. J. Immunol., Oct 2009, 183: 4475 - 4482.
2. J. Immunol., Oct 2009, 183: 4619 - 4627.
3. J. Exp. Med., Sep 2009, 206: 2235 - 2251.

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Mouse Interleukin-10 Recombinant

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