Human Interleukin-1 beta Recombinant

Catalog #
90168-A
$130 *
Size: 2 µg
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

Recombinant IL-1 beta is a disulfide-linked monomer protein consisting of two 154 amino acid residues. Recombinant IL-1 beta migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-1 beta mature chain was expressed in E. coli.

Synonyms
IL-1B, IL-1beta, Interleukin-1 beta, Catabolin
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
E. coli
Purity
≥97% by SDS-PAGE and HPLC
Format
lyophilized protein
Formulation
Lyophilized from 0.2 µm filtered PBS, pH 7.0.
MW
17 kDa
Endotoxin Level
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Amino Acids
117–269
Biological Activity
The ED50 as determined by the dose-dependent proliferation of murine D10S cells, was found to be ≤ 2.0 pg/ml, corresponding to a specific activity of >2 x 109 units/mg
Genbank #
P01584
UniProt #
P01584
Background
Monocytes are the main source of secreted IL-1. They express predominantly IL-1beta while human keratinocytes express large amounts of IL-1alpha. Murine macrophages display a transition from IL-1beta to IL-1alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL-1, IL-1alpha and IL-1beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL-1alpha in mice. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1beta but not the IL-1alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.
References
1. J. Pharmacol. Exp. Ther., Oct 2009, 331: 104 - 113.
2. J. Endocrinol., Oct 2009, 203: 55 - 63.
3. Nephrol. Dial. Transplant., Sep 2009, 24: 2655 - 2665.