IKCA1 (KCNN4) HEK293 Cell Line

Catalog #

90330

$9,000 *

Size: 2 vials

Qty

Bulk/Custom

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Description

IKCA1 (KCNN4) HEK293 Cell Line are engineered HEK293 cells expressing human IKCA1, also known as KCNN4 (Intermediate conductance calcium-activated potassium channel protein 4), IK1, hKCa4, and hSK4), Genbank Accession No. NM_002250. This cell line has been validated in a cellular assay using a membrane potential-sensitive fluorescence dye (DiBAC4(3)) and by Western blot for IKCA1 expression.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

IKCA1, KCNN4, Intermediate conductance calcium-activated potassium channel protein 4, IK1, hKCa4, hSK4

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Product Data Gallery

Ionomycin-induced increase of intracellular calcium level activates IKCA1 channel expressed in IKCA1 (KCNN4) HEK293 Cell Line

Ionomycin-induced IKCA1 activation in the IKCA1 (KCNN4) HEK293 Cell Line is blocked by clotrimazole (CLT), an IKCA1 channel inhibitor

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.

Species

Human

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1B	BPS Bioscience #79531

Biological Activity

N-terminal FLAG-tagged human IKCA1 has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. Functional analysis of IKCA1 channel in the stable recombinant HEK293 was accomplished by using the membrane potential–sensitive fluorescence dye DiBAC4(3). When IKCA1-expressed HEK293 cells were stimulated with calcium-specific ionophore ionomycin to raise the intracellular level of Ca²⁺, IKCA1 channel was activated, leading to hyperpolarization and a decrease in cell fluorescence in the functional fluorescence assay with DiBAC4(3). This decrease of cell fluorescence was blocked by the IKCA1 channel inhibitor, clotrimazole (CLT).

Genbank #

NM_002250

UniProt #

O15554

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

IKCA1, also known as Intermediate conductance calcium-activated potassium channel protein 4), IK1, hKCa4, and hSK4, are part of a potentially heterotetrametric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes, but it is also present in endothelial cells and cardiac fibroblasts. They contribute to vascular smooth muscle cell proliferation, migration and cardiac fibrosis. IKCA1 was found to be present at high levels in breast cancer (BC) cells, and when depleted lead to decreased tumorigenesis. IKCA1 can be modulated with small molecules, and these may provide a therapeutical opportunity in the treatment of BC and cardiovascular diseases.

References

Ghanshani S., et al., J Biol Chem. 275(47): 37137-37149.

Hoffman J.F., et al., 2003 PNAS 100 (12): 7366-7371.

de-Allie F.A., et al., 1996, Br J Pharmacol. 117(3):479-487.

Terstappen G.C., et al., 2003 Neurosci Lett. 346(1-2):85-88.

Gross D., et al., 2022 Cell Death and Disease 13:902.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1B (BPS Bioscience #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 μg/ml of Geneticin to ensure the recombinant expression is maintained. IKCA1-HEK293 cells typically exhibit a cell division time of ~24 hours.

Applications

Screen and validate agonists and antagonists of IKCA1 during research and drug discovery.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ion Channel, Neuroscience

Regulatory Status

Research Use Only