Mouse Interferon-gamma Recombinant
Catalog #
90163-A
$130
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Description
Recombinant Interferon gamma is a disulfide-linked homodimeric protein consisting of 134 amino acid residues, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding murine Interferon-gamma mature chain was expressed in E. coli.
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Synonyms
IFNG, Interferon gamma, IFG, IFN
Product Info
Storage and Usage
Citations
Species
Mouse
Host Species/Expression System
E. coli
Purity
≥95% by SDS-PAGE and HPLC
Format
lyophilized protein
Formulation
Lyophilized from 0.2 µm filtered PBS.
MW
16 kDa
Endotoxin Level
Endotoxin level was found to be < 0.1 ng/μg (1 EU/μg), using the LAL gel clot method.
Biological Activity
The ED50 was determined by the cytopathic inhibition assay with murine L929 cells challenged with EMC virus to be 0.1 ng/ml
UniProt #
P01580
Background
IFN-gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFN gamma receptors are expressed on all types of human cells with the exception of mature erythrocytes. IFN-gamma-receptor complexes are rapidly internalized by endocytosis. IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. Interferon-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types. The growth inhibitory activities of IFN-gamma are more pronounced than those of the other interferons. However, the main biological activity of IFN-gamma appears to be immunomodulatory in contrast to the other interferons that are mainly antiviral. In T-helper cells, IL-2 induces the synthesis of IFN-gamma and other cytokines. IFN- gamma acts synergistically with IL-1 and IL-2 and appears to be required for the expression of IL-2 receptors on the cell surface of T-lymphocytes. Blocking of the IL-2 receptor by specific antibodies also inhibits the synthesis of IFN-gamma.
References
1. Sikorski K, et al. Cytokine Growth Factor Rev. 2011 Aug,22(4):211-9.
2. Gysemans C, et al. Biochem Soc Trans. 2008 Jun,36(Pt 3):328-33.
3. Rameshwar P. Cell Res. 2008 Aug,18(8):805-6.
2. Gysemans C, et al. Biochem Soc Trans. 2008 Jun,36(Pt 3):328-33.
3. Rameshwar P. Cell Res. 2008 Aug,18(8):805-6.
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