IDO2 Inhibitor Screening Assay Kit
The IDO2 Inhibitor Screening Assay Kit is designed to measure IDO2 enzyme inhibition. The IDO2 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate.
| Catalog Number |
Component |
Amount |
Storage |
|
| 71194 | IDO2 His-Tag | 1 x 500 µg | -80°C | Avoid freeze/ thaw cycles! |
| IDO2 Reaction Solution component 1 | 2 x 10 ml | -80°C | ||
| IDO2 Reaction Solution component 2 | 2 x 100 µl | -80°C | ||
| IDO2 Substrate | 2 x 1 ml | -80°C | ||
| 1x IDO2 Assay Buffer | 5 ml | -80°C | ||
| UV transparent 96-well plate | 1 | Room Temp. | ||
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
2. Seegers, N., et al. J. Biomol. Screen. 2014; 19(9): 1266-74.
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