IDO2 - HEK293 Recombinant Cell Line (Human)

Catalog #
60533
$10,340 *
Size: 2 vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

Recombinant HEK293 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO2), Genbank accession number NM_194294.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
Indoleamine 2, 3-dioxygenase 2, IDO2, IDO
Product Info
Storage and Usage
Citations
Host Cell Line
HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Species
Human
Supplied As
Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Materials Required But Not Supplied

Materials Required for Cell Culture

Name Ordering Information
Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1D BPS Bioscience #79536

 

Materials Required for Cellular Assay

Name Ordering Information
Thaw Medium 1 BPS Bioscience #60187
Growth Medium 1D BPS Bioscience #79536
Doxycycline MP Bio, #0219504401
INCB024360 BPS Bioscience #27339
6.1 N Trichloroacetic acid Sigma #T0699
Acetic acid Sigma #695092
IDO2 Cellular Activity QuickDetect™ Supplements BPS Bioscience #62001
96-well tissue culture treated assay plate  
96-well plate or tube for thermocycler  
Genbank #
NM_194294
UniProt #
Q6ZQW0
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

References

1. Metz, R., et al., Int. Immunol. 2014; 26: 357–367.

2. Fatokun, A., et al., Amino Acids 2013; 45: 1319-1329.