IDO2 - HEK293 Recombinant Cell Line (Human)
Recombinant HEK293 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO2), Genbank accession number NM_194294.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Materials Required for Cell Culture
Name | Ordering Information |
Thaw Medium 1 | BPS Bioscience #60187 |
Growth Medium 1D | BPS Bioscience #79536 |
Materials Required for Cellular Assay
Name | Ordering Information |
Thaw Medium 1 | BPS Bioscience #60187 |
Growth Medium 1D | BPS Bioscience #79536 |
Doxycycline | MP Bio, #0219504401 |
INCB024360 | BPS Bioscience #27339 |
6.1 N Trichloroacetic acid | Sigma #T0699 |
Acetic acid | Sigma #695092 |
IDO2 Cellular Activity QuickDetect™ Supplements | BPS Bioscience #62001 |
96-well tissue culture treated assay plate | |
96-well plate or tube for thermocycler |
The cell line has been screened to confirm the absence of Mycoplasma species.
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
1. Metz, R., et al., Int. Immunol. 2014; 26: 357–367.
2. Fatokun, A., et al., Amino Acids 2013; 45: 1319-1329.