IDO1 Inhibitor Screening Assay Kit -384

Catalog #
72034
$1,070 *
Size: 384 reactions
Qty
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Description

The IDO1 Inhibitor Screening Assay Kit is designed to measure IDO1 enzyme inhibition. The IDO1 Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at  =320–325 nm.

Synonyms
Indoleamine 2,3-dioxygenase 1, IDO, IDO1 Assay Kit, activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Colorimetric - UV Spectrophotometry
Supplied As
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions.
Format

COMPONENTS:

UniProt #
P14902
Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
References

1. Liu, X., et al., Blood 2010; 115(17): 3520-3530.
2. Seegers, N., et al. J. Biomol. Screen. 2014; 19(9): 1266-74.