IDH2, FLAG-tag Recombinant

Catalog #

71074-2

$505 *

Size: 100 µg

Qty

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Description

Human isocitrate dehydrogenase 2, also known as IDH2, (GenBank Accession No. NM_002168), a.a. 1-452(end) with C-terminal FLAG-tag, MW = 52 kDa, expressed in a baculovirus infected Sf9 cell expression system.

This product has been cited 2 times.

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Synonyms

Isocitrate Dehydrogenase 2, IDH2, oxalosuccinate decarboxylase, IDH-2

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥59%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 3 mM DTT and 10% glycerol.

52 kDa

Amino Acids

1–452(end)

Specific Activity

≥14,200 pmol/min/µg.

Genbank #

NM_002168

UniProt #

P48735

Tag(s)

C-terminal FLAG-tag

References

1. Schaap, F.G., et al., Adv Anat Pathol 2013;20(1):32-38.
2. Grassian, A.R., et al., J Biol Chem 2012;287(50):42180-42194.

Application Reference(s):
1. Identification of a selective inhibitor of IDH2/R140Q enzyme that induces cellular differentiation in leukemia cells (2020)

Storage/Stability

>6 months at –80°C

Assay Conditions

The reaction mixture contained 50 mM Tris, pH 7.4, 5 mM MgCl₂, 200 µM DL-isocitrate, 200 µM NADP+, and various amount of IDH2 in a volume of 200 µL. Reactions were initiated with the addition of IDH2, and the increase in NADPH absorbance at 340 nm was continuously monitored for 7 min. Molar extinction coefficient of NADPH is 6,220 M^-1cm^-1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Dehydrogenase

Regulatory Status

Research Use Only

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Datasheets/Protocols

71074_Lot110106-2.pdf

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IDH2, FLAG-tag Recombinant

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