LIGHT-Responsive HVEM/NF-κB Reporter Jurkat Cell Line

Catalog #

79310

$11,095 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

LIGHT-Responsive HVEM/NF-κB Reporter Jurkat Cell Line is a Jurkat cell line designed for monitoring HVEM (Herpes virus entry mediator) related NF-κB (nuclear factor κB) signal transduction pathways. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element and expresses human HVEM. After activation by the HVEM ligand LIGHT, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter.

HVEM LIGHT Cell Line

Figure 1: Mechanism of action of LIGHT-Responsive HVEM/NF-κB Jurkat Cell Line in response to LIGHT.
LIGHT, either as a soluble ligand or when present on the cell surface of a cell, binds to HVEM, and NF-ĸB transcription factors is activated, inducing transcription of the luciferase reporter.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of HVEM without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

HVEM, TNFRSF14, CD270, HVEA, TR2, LIGHTR, HVEM Cell line, HVEM NFAT, nfkb, nf-kb

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Product Data Gallery

HVEM expression in LIGHT-Responsive HVEM/NF-κB Reporter Jurkat Cell Line analyzed by flow cytometry

LIGHT-Responsive HVEM/NF-κB Reporter Jurkat Cell Line activation by soluble and membrane bound LIGHT

Dose response curves of LIGHT-Responsive HVEM/NF-κB Reporter Jurkat Cell Line to soluble and membrane bound LIGHT

Inhibition of LIGHT-induced HVEM/NF-κB activity by the NF-κB inhibitor IKK-16 dihydrochloride, in LIGHT-Responsive HVEM/NF-κB Reporter Jurkat Cell Line

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat cells (clone E6-1), Human T lymphoblast, suspension.

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2A	BPS Bioscience #60190

Materials Required for Cellular Assay

Name	Ordering Information
Assay Medium: Thaw Medium 2	BPS Bioscience #60184
LIGHT, His-Tag (Human) Recombinant	BPS Bioscience #71266
LIGHT-CHO Recombinant Cell Line	BPS Bioscience #79262
IKK-16 dihydrochloride: inhibitor of NF-κB activation	Sigma #SML1138
NF-κB Luciferase Reporter Jurkat Cell Line	BPS Bioscience #60651
96-well tissue culture-treated white clear-bottom assay plate	Corning #3610
ONE-Step™ luciferase assay system	BPS Bioscience #60690
Luminometer

UniProt #

Q92956

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

HVEM (Herpes virus entry mediator), also known as CD270 or TNFRSF14, is a human cell surface receptor of the TNF-receptor superfamily that can act as both a co-stimulatory and a co-inhibitory receptor of T cells. Binding of HVEM to one of its ligands, LIGHT (CD258, TNFSF14) or LTα (lymphotoxin-α), causes a co-stimulatory signal which can activate lymphoid cells. Alternately, interaction with BTLA (CD272) or CD160 causes a co-inhibitory signal which negatively regulates T-cell immune responses. HVEM has also been shown to interact with the adaptor proteins TRAF2 and TRAF5 and is critical to herpes simplex virus (HSV) cellular entry. LIGHT, either in the soluble form or as oligomers expressed on the cell surface, can activate NF-κB signaling pathway through binding to HVEM. The LIGHT/HVEM axis are co-stimulatory immune checkpoint molecules extensively studied in cancer immunotherapy.

References

Chen L. and Flies D., 2013 Nat Rev Immunol. 13(4): 227–242.
Steinberg M., et.al. 2014 Immunol Rev. 244(1): 169–187.
Rio M., et.al. 2014 Transplantation. 15; 98(11): 1165–1174.
Shui W., et.al. 2014 Immune Netw. 14(2): 67–72.
Steinberg M., et.al. 2009 Semin. Immunopathol. 31: 207-221.
Ware C., 2009 Adv.Exp.Med.Biol. 647:146.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. To formulate a comparable but not BPS validated media, formulation components can be found below.

Media Required for Cell Culture
Thaw Medium (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2A (BPS Bioscience #60190): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml of Geneticin, and 200 μg/ml of Hygromycin B.

Media Required for Functional Cellular Assay
Thaw Medium (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Applications

Screen for agonists or antagonists of LIGHT-HVEM signaling in a physiologically relevant cellular model.
Characterize T cell-mediated immune responses of HVEM and its interactions with LIGHT.
Screen co-stimulatory immune checkpoint molecules for cancer immunotherapy.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only