CD226, Fc fusion, Biotin-labeled (Human) HiP™ Recombinant

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Catalog #

71253

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Description

Human CD226 antigen also known as DNAX accessory molecule 1 (DNAM-1), GenBank Accession No. NM_006566, a.a. 19-247 fused to Fc region of human IgG1, this protein is enzymatically biotinylated using AviTag^TM technology and expressed in a HEK293 cell expression system. MW = 54.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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Synonyms

DNAX accessory molecule 1 (DNAM-1)

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD226:CD155 Assay

Gel Filtration Curve

CD226:CD112 inhibition curve using an AlphaLISA assay (#72051). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

54.7 kDa

Amino Acids

19-247

Genbank #

NM_006566

UniProt #

Q15762

Tag(s)

C-terminal Fc fusion (Human IgG1), AviTag™

References

1. Bottino, C., et al., J. Exp. Med. 2003; 198(4): 557-567.
2. Hafler, J., et al., Genes Immun. 2009; 10: 5-10.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

CD226, biotin binding to CD155, His-tag was confirmed according to the protocol for BPS Cat. 72052. Briefly, 6 ng of CD226-biotin was mixed with varying amounts of CD155, His-tag in CD226 assay buffer at a final volume of 10 µl and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 min, followed by Strep-conjugated acceptor beads for 30 min and reading alpha-counts.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and for screening small molecules and antibodies.

Shipping Temperature

dry ice

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to 100 μg/ml in PBS + glycerol and stored at - 80°C.

Scientific Category

Immunotherapy/Cell Surface Receptor

Regulatory Status

Research Use Only

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Datasheets/Protocols

71253_Lot160314-G.pdf

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CD226, Fc fusion, Biotin-labeled (Human) HiP™ Recombinant

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