TMEM16B (ANO2) - HEK293 Recombinant Cell line

Catalog #

90332

$9,400 *

Size: 2 vials

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Description

Recombinant HEK293 cell line expressing human TMEM16B (transmembrane protein 16B, also called as anoctamin 2, calcium-activated chloride channel (ANO2), accession number NM_001278596).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

TMEM16B, transmembrane protein 16B, anoctamin 2, calcium-activated chloride channel, ANO2

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Product Data Gallery

TMEM16B expressed in HEK293 produced I- influx after extracellular addition of I- with ionomycin. A) TMEM16B-HEK293; B) parental HEK293 cells

Ionomycin-induced I- influx in TMEM16B-HEK293 cells was blocked by niflumic acid, a CaCC channel blocker. A) TMEM16B-HEK293; B) parental HEK293 cells

Whole- cell patch clamp recordings from HEK-hTMEM16B cells

Niflumic acid blocks the currents in HEK-hTMEM16B cells

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293 cells

Species

Human

Supplied As

Each vial contains ~1.5 X 10⁶ cells in 1 ml of 10% DMSO.

Biological Activity

Human TMEM16B channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The CaCC activity of TMEM16B was characterized by an assay based on a halidesensitive yellow fluorescent protein (YFP) mutant whose fluorescence is quenched by increasing halide concentration. When TMEM16B-expressed HEK293 cells were stimulated with ionomycin to raise the intracellular level of Ca²⁺, TMEM16B produced I- influx in HEK293, triggering a rapid decrease of fluorescence by the transfected YFP mutant. The ionomycin-induced I- influx through TMEM16B was blocked by niflumic acid, a CaCC channel blocker.

Genbank #

NM_001278596

UniProt #

Q9NQ90

Mycoplasma Testing

The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.

Background

Calcium-activated chloride channels (CaCCs) are involved in a variety of physiological functions including smooth muscle contraction and olfaction. TMEM16B (ANO2) has been identified as a CaCC that is activated by intracellular Ca²⁺ and Ca²⁺-mobilizing stimuli. It has eight putative transmembrane segments and is permeable to monovalent anions.

References

1. Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. Nature Reviews 8:153-171.

2. Scudieri P. et al. (2012) The anoctamin family: TMEM16A and TMEM16B as calciumactivated chloride channels. Exp. Physiol. 97(2):177-183.

3. Stöhr H. et al. (2009) TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals. J. Neurosci. 29(21):6809-6818.

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Cat. #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1F (BPS Cat. #79540): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 100 µg/ml of Hygromycin B to ensure the recombinant expression is maintained. It may be necessary to adjust the percentage of CO2 in the incubator, depending on the NaHCO3 level in the basal medium. TMEM16B-HEK293 cells should exhibit a typical cell division time of approximately 24 hours.

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Applications

• Drug compound screening for activators or inhibitors of TMEM16B
• Functional assays for TMEM16B
• Efficient antigen for mouse immunization

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ion Channel, Neuroscience

Regulatory Status

Research Use Only

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TMEM16B (ANO2) - HEK293 Recombinant Cell line

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