• TDO Cellular Activity QuickDetectTM Supplements (BPS Cat. #62002). Note: other formulations can be used, but significant optimization may be required.
UniProt #
P48775
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit
(Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Background
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in
mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key
regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an
immunosuppressive microenvironment by starving immune cells of L-Trp and releasing
degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3
dioxygenase (TDO), is upregulated in many tumors, providing cancer cells with an avenue for
immune evasion.
References
1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
Storage/Stability
Immediately upon receipt, store vials in liquid nitrogen
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS Cat. #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1B (BPS Cat. #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml of Geneticin to ensure that recombinant expression is maintained. hTDO-HEK293 cells should exhibit a typical cell division time of ~33 hours.
Applications
Monitor TDO pathway activity. Screen for activators or inhibitors of TDO in a cellular context
Shipping Temperature
-80°C (dry ice)
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.
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