HSP90α (C-Terminal Domain) TR-FRET Kit

Catalog #: 50261
Size: 384 reactions
$1,040
Description:
The HSP90α (C-Terminal Domain) TR-FRET assay kit is designed to measure the inhibition of HSP90α binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a homogeneous, 384 reaction format. This assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90α, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by measuring the TR-FRET between the PPID and HSP90α using a fluorescence reader.
UniProt
P07900
Synonym(s):
HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α
Supplied As:
Kit comes in a homogeneous, 384 reaction format
Format:
Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

Allan, R.K. et al. J. Biol. Chem. 2006 281(11): 7161-71

Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance
Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Heat Shock Proteins/Assay Kits
Product Type:
Assay Kits
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50261.pdf