HSP90α N-Terminal Domain Assay Kit

Catalog #
50293
$530 *
Size: 96 reactions
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Description

The HSP90α Assay Kit is designed for identification of HSP90α inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90α. The key to the HSP90α Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90α reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90α enzyme to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

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This product has been cited 24 times.

Synonyms
HSP90a, Heat Shock Protein 90a, HSP90alpha, HSP90α
Product Info
Storage and Usage
Citations24
Assay Kit Format
Fluorescence Polarization
Supplied As
The HSP90α inhibitor screening assay kit comes in a convenient 96-well format, with purified HSP90α enzyme, fluorescently labeled geldanamycin and HSP90α assay buffer for 96 enzyme reactions.
Format
Catalog
Number

Component

Amount

Storage
50290 Hsp90α recombinant enzyme 100 µg -80°C


Avoid
freeze/
thaw
cycles! 
50312 FITC-labeled geldanamycin (2.5 µM) 30 µl -80°C
50311 5x Hsp90 Assay Buffer 1 4 ml -20°C
  Black, low binding NUNC microtiter plate 1 Room
Temp
UniProt #
P07900
Background

Hsp90α is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90α function has been shown to play a role in tumorigenesis and disease progression.

References

1. Kim, J., et al. J Biomol Screening 2004, 9(5): 375-381.
2. Howes, R., et al. Anal Biochem 2006, 350:202-213.