The HSP90α Assay Kit is designed for identification of HSP90α inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant HSP90α. The key to the HSP90α Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for HSP90α reactions. The fluorescently labeled geldanamycin is incubated with a sample containing HSP90α enzyme to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.
The HSP90α inhibitor screening assay kit comes in a convenient 96-well format, with purified HSP90α enzyme, fluorescently labeled geldanamycin and HSP90α assay buffer for 96 enzyme reactions.
Hsp90α is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90α function has been shown to play a role in tumorigenesis and disease progression.
References
1. Kim, J., et al. J Biomol Screening 2004, 9(5): 375-381. 2. Howes, R., et al. Anal Biochem 2006, 350:202-213.
Storage/Stability
Stable for at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See data sheet for detailed protocol.
Applications
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
-80°C
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.