The HSP90β (C-terminal) Inhibitor Screening Assay Kit is designed to measure the inhibition of HSP90β binding to its protein target PPID (also known as Cyclophilin D). With this kit, only three simple steps on a microtiter plate are required. First, a sample containing HSP90β, PPID, and an inhibitor of choice is incubated for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Kit comes in a convenient AlphaLISA® format, with enough HSP90β (527-724), assay buffer, detection buffer, and purified GST-tagged PPID to perform a total of 384 enzyme reactions.
Format
COMPONENTS:
UniProt #
P08238
References
Allan, R.K. et al. J. Biol.Chem 2006 281(11): 7161-71.
Storage/Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Applications
Useful for screening inhibitors of HSP90β and for HSP90β binding assays
Shipping Temperature
-80°C
Notes
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Contraindications
Avoid DMSO concentrations above 0.5%. Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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