HSP90β (C-terminal), Biotin-Labeled, His-Tag, Avi-Tag Recombinant

Catalog #

50313

$475 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human HSP90β (Human Heat Shock Protein 90 beta), encompassing amino acids 527-724(end). This construct contains an N-terminal His-tag (6xHis) followed by an Avi-tag™. The recombinant protein was enzymatically biotinylated at the C-terminal using Avi-Tag technology and affinity purified. Biotinylation is confirmed to be ≥90%.

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Synonyms

Hsp90beta, heat shock protein 90 beta

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

HSP90β [Biotinylated]: PPID-GST Activity

Biotin-Avidin Pulldown

HSP90ß (C-Terminal Domain) inhibition curve using a TR-FRET assay (#50262-2). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

HSP90β (C-terminal) (His-Avi-527-724(end))-(Biotin)

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol

25 kDa

Amino Acids

527-724 (end)

Genbank #

NM_001271969

UniProt #

P08238

Tag(s)

N-terminal His-tag, N-terminal Avi-tag™

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

References

1. Milicevic, Z. et al., Int. J. Oncol. 2008 Jun;32(6):1169-78.
2. Chao, C.C. et al., J. Cell Biochem. 2008 Jul 1;104(4):1286-96.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was done according to HSP90β (C-terminal) Inhibitor Screening Kit (BPS Bioscience #50314) with HSP90β[B] at 35 ng/rxn and PPID-GST at 52.5 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Heat Shock Proteins

Regulatory Status

Research Use Only