HSP90α C-Terminal Domain TR-FRET Assay Kit

Catalog #: 50289
Size: 96 reactions
This assay kit is designed to measure the inhibition of the HSP90α binding to its protein target PPID (also known as Cyclophilin D) in a homogeneous, 96 reaction format. This assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90α, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by measuring the TR-FRET between the PPID and HSP90α using a fluorescence reader.
HSP90, Assay kit, HSP90a, HSP90α, Fret assay kit, HTRF

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
At least 6 months from date of receipt when stored as directed.
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Allan, R.K. et al. J. Biol. Chem 2006 281(11): 7161-71
Avoid freeze/thaw cycles.
Scientific Category:
Heat Shock Proteins
Product Type:
Assay Kit
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