HRV 3C Protease, His-GST-tags Recombinant

Catalog #

50319

$325 *

Size: 1000 Units

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human rhinovirus 3C protease (HRV 3C Protease) with Nterminal His-GST-tags (can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag.), Recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. Cleaves between Gln and Gly. MW = 47 kDa in E. coli expression system.

●

Synonyms

Human rhinovirus 3C protease, HRV3C

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity. *Not all lots have been tested for enzyme activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

HRV 3C Protease (His-GST-1538-1717)

Host Species/Expression System

E. Coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM TCEP, 50% glycerol

Concentration

1.90 mg/ml

47 kDa

Amino Acids

1538-1717

Specific Activity

1 Unit of HRV 3C Protease cleaves >95% of 100 μg of target protein at 4°C for 16 hours.

UniProt #

P03303

Tag(s)

N-terminal His-GST-tags

References

1. Timmer JC, Salvesen GS. Methods Mol Biol. 2011;753:243-55.

Storage/Stability

At least 6 months at -80°C

Assay Conditions

A 68 kDa GST-fusion protein (C) at a ratio of (1) 1:100, (2) 1:200, (3) 1:400, (4) 1:800 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM β-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Protease

Regulatory Status

Research Use Only