Hrd1, SUMO-His-tags

Catalog #: 81051
Size: 25 µg
Species: Human
Host Species: E. coli
MW: 39.3 kDa (without SUMO tag)
Genbank #: Q86TM6.2
Tag(s): N-terminal SUMO-His-tags
a.a: 236–617
Hrd1 is a RING domain E3 ligase that is involved in the conjugation of ubiquitin to target substrates. Hrd1 has been demonstrated to function with the E2 enzymes UBE2D3 (UbcH5c) and UBE2G2 in vitro. Hrd1 is an ER-associated ligase involved in ERAD and has been linked to rheumatoid arthritis. It is present in human rheumatoid synovial cells and found to be a causative factor for arthropathy in in vivo studies. Hrd1 is also found in brain neurons and its proper regulation may be linked to neurodegenerative diseases. This construct is a Hrd1 N-terminal deletion containing residues 246-617 consisting of the complete C-terminal cytoplasmic portion. The N-terminal transmembrane spanning portion has been deleted. This Hrd1 N-terminal deletion mutant is active as an E3 ligase containing the RING and substrate recognition domains.
Cytoplasmic region of human Hrd1 recombinant protein (Genbank Accession No. ), a.a. 236-17, with N-terminal SUMO-tag, expressed in E. coli.
E3 ubiquitin-protein ligase synoviolin, HRD1, KIAA1810, MGC40372, Synovial apoptosis inhibitor 1
≥85% by SDS-PAGE
Biological Activity:
Typical enzyme concentration of 20-1000 nM is used for in vitro conjugation depending on assay conditions.
PBS, 10% glycerol
Aqueous buffer solution
Storage / Stability:

Stable for ≥1 year at –80°C.

Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
1. Kaneko, M., et al. Molecular approaches to the treatment, prophylaxis, and diagnosis of Alzheimer's disease: possible involvement of HRD1, a novel molecule related to endoplasmic reticulum stress, in Alzheimer's disease. J. Pharmacol. Sci, 2012. 118(3):325-30.
2. Yagishita, N., et al. Synoviolin, protein folding and the maintenance of joint homeostasis, Nat. Clin. Prac. Rheumatol, 2008. 4(2):91-7.
3. Yamasaki, S., et al. The roles of synoviolin in crosstalk between endoplasmic reticulum stress-induced apoptosis and p53 pathway. Cell Cycle 6(11):1319-23.
Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitination (Ubiquitin Ligase (E3))
Product Type:
Recombinant Protein
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