The Homogeneous Caspase-6 Assay Kit is a complete assay system designed to measure Caspase-6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-6 activity measurements. In addition, the kit includes purified Caspase-6 enzyme and a potent Caspase-6 inhibitor, Ac-IETD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-6 activity level. The fluorogenic substrate, Ac-VEID-AFC, is incubated with purified Caspase-6 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.
1. Graham R.K., et al., Trends Neurosci. 2011 Dec;34(12):646-56 2. Richards S., et al., Immunol Rev. 2008 August; 224: 183–200.
Storage/Stability
6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Useful for studying enzyme kinetics and HTS applications.
Shipping Temperature
-80°C
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.