Human recombinant histone octamer consisting of 2 molecules each of histones H2A encompassing amino acids 2-130(end), H2B encompassing amino acids (2-126(end), H3 encompassing 2-136(end), and H4 encompassing amino acids 2-103(end). Each recombinant histone has an N-terminal His-tag (6xHis). The recombinant complex was affinity purified.
The histone octamer is composed of a central heterotetramer of two copies of both histones H3 and H4, flanked by two heterodimers of histones H2A and H2B. The octamer assembles when the H3/H4 tetramer complexes with two H2A/H2B dimers. These histones of the histone octamer all contain N-terminal tails that emanate from their central histone folds and are targets for epigenetic modification.
References
1. Miller KM, Jackson SP. Biochem Soc Trans 40(2):370-6 (2012). 2. Raut VV, Pandey SM, Sainis JK. Ann Bot 108(7):1235-46 (2011). 3. Park K, Fasman GD. Biochemistry. 1987 Dec 15;26(25):8042-5. 4. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20,5(1):11.
Storage/Stability
At least 6 months at -80°C.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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