Histone H4(R3) Methyltransferase Assay Kit

Catalog #

52074

$850 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Histone H4(R3) Universal Methyltransferase Assay Kit is designed for the detection of histone H4 (R3) methyltransferase (HMT) activity using purified enzymes or cell extracts. Histone H4 (R3) methyltransferases are enzymes that catalyze the transfer of a methyl group from the cofactor S-adenosylmethionine to arginine 3 residue of histone H4.
The key to the Histone H4(R3) Universal Methyltransferase Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

The Histone H4(R3) Universal Methyltransferase Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine residue of Histone H4, the secondary HRP-labeled antibody, S-

Materials Required But Not Supplied

TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog #	Components	Amount	Storage
51040	PRMT1 human recombinant enzyme*	1 µg	-80°C	*Avoid freeze/ thaw cycles!*
51043	PRMT3 human recombinant enzyme	10 µg	-80°C
51045	PRMT5 human recombinant enzyme	10 µg	-80°C
	20 µM S-adenosylmethionine	250 µl	-80°C
52150	Primary antibody 4	100 µl	-80°C
52150-3	Primary antibody 4-3	100 µl	-80°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
52170	4x HMT Assay Buffer 2	3 ml	-20°C
52100	Blocking buffer 4	50 ml	+4°C
	HRP chemiluminescent substrate (2 components)	6 ml each	+4°C
	96-well plate precoated with histone substrate	1 plate	+4°C

*We have provided additional material for ease of retrieval.

UniProt #

PRMT1: Q99873; PRMT3: Q8WUV3; PRMT5: O14744

References

Dillon S.C., et al. 2005. Genome Biology 6:227.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only

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Histone H4(R3) Methyltransferase Assay Kit

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