Histone H3, Full Length, His-tag

Catalog #: 52023
Size: 1 mg
$450
Species: Human
Host Species: E. coli
MW: 15.4 kDa
Genbank #: NM_003532
Tag(s): N-terminal His-tag
a.a: 2–136(end)
Description:
Human Histone 3 (HIST1H3E), GenBank Accession No. NM_003532, a.a. 2-136(end) with N-terminal His-tag MW = 15.4 kDa, expressed in an E. coli expression system.
UniProt
P68431
Synonym(s):
Histone 3.1, HIST1H3E
Assay Conditions:
Histone H3.1 was coated onto a 96-well plate, and methylation by G9a was carried out at room temperature for 60 min. Methylated Histone H3.1 was detected using Chemiluminescence assay.
Formulation:
8 mM PBS pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at –80°C.

Application(s):
Useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.
Reference(s):

1.  Loyola, A., et  al., Mol. Cell.  24(2), 309-316 (2006). 
2.  Hans, F. and  Dimitrov,  S.,  Oncogene. 20(24), 3021-3027 (2001). 

Product Specific Reference(s):
1. Morita, S., et al., Peptides. 48, 75-82 (2013). 

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase/Substrate
Product Type:
Recombinant Protein
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Product Datasheets

52023_Lot131122.pdf