CD73 His-tag (Human) HiP™

Catalog #: 71184
Size: 50 µg
$300
Species: Human
Host Species: HEK293
MW: 58.6 kDa
Genbank #: NM_002526
Tag(s): C-terminal His-tag
a.a: 27-547
Background:

CD39 contributes to immune regulation through the hydrolysis of ATP to AMP in the rate limiting step of extracellular adenosine production. CD39 is expressed on the surface of multiple cells of the immune system including T-cells, Natural killer cells, B-cells, dendritic cells and macrophages. In addition, high levels of CD39 expression have been associated with the tumor microenvironment (TME). Research aimed at reducing immunosuppression by CD39 activity has resulted in the study of a small molecule inhibitor, POM1, and multiple anti-CD39 antibodies selected for blocking enzymatic activity or decreasing CD39 expression. 

Description:

Human CD73 protein, also known as 5'- nucleotidase and Ecto-5'-nucleotidase, GenBank Accession No. NM_002526, a.a. 27-547 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 58.6 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt
P21589
Synonym(s):
5'- nucleotidase, Ecto-5'-nucleotidase, CD73, CD73 Protein
Specific Activity:
≥ 20 nmol/min/μg
Assay Conditions:
Assay was done in 50 µL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 µM AMP for 12 min at 37º C. Inorganic phosphate produced was measured using Biomol Green reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Reference(s):
1. Deaglio, S. et al., J. Exp. Med. 2007; 204(6): 1257-1265.
2. Thompson, L.F., et al., J. Exp. Med. 2004; 200(11): 1395-1405.

Notes:

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warning(s):
Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Immunotherapy
Product Type:
Recombinant protein
Data shown is lot-specific. Contact us for specific information on other lots.

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