CD73 His-tag (Human) HiP™

Catalog #: 71184
Size: 50 µg
$300.00
Species: Human
Host Species: HEK293
MW: 58.6 kDa
Genbank #: NM_002526
Tag(s): C-terminal His-tag
a.a: 27-547
Description:

Human CD73 protein, also known as 5'- nucleotidase and Ecto-5'-nucleotidase, GenBank Accession No. NM_002526, a.a. 27-547 with C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 58.6 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt
P21589
Synonym(s):
5'- nucleotidase, Ecto-5'-nucleotidase, CD73, CD73 Protein
Specific Activity:
≥ 20 nmol/min/μg
Assay Conditions:
Assay was done in 50 µL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 µM AMP for 12 min at 37º C. Inorganic phosphate produced was measured using Biomol Green reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Reference(s):
1. Deaglio, S. et al., J. Exp. Med. 2007; 204(6): 1257-1265.
2. Thompson, L.F., et al., J. Exp. Med. 2004; 200(11): 1395-1405.

Notes:

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warning(s):
Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Immunotherapy
Product Type:
Recombinant protein
Data shown is lot-specific. Contact us for specific information on other lots.

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