B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP™

Catalog #: 71114
Size: 50 µg
$405.00
Species: Human
Host Species: HEK293
MW: 52.5 kDa
Genbank #: NM_005191
Tag(s): C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
a.a: 35-242
Description:

Human secreted B7-1-Fc fusion protein, also known as CD80, with C-terminal Avi-tag™. GenBank Accession No. NM_005191, a.a 35-242 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 52.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

UniProt
P33681
Synonym(s):
CTLA-4 Counter-Receptor B7.1
Assay Conditions:
Reaction done according to CTLA4:B7-1[biotin] assay kit (Catalog # 72009) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein was added ON at 4 deg 100 ng/well. Various concentrations of biotinylated ligand (0-8ng/ul, 0.5 ng/ul is the recommended kit condition) added for 2h at RT. Strep-HRP added for 1h at RT.
Formulation:
40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Reference(s):

1. Byrum, J.N., Van Komen, J.S., Rodgers, W., J. Immunol. 2013 Sep 15;191(6):3073-3081.

2. Legat, A., et al., Front Immunol. 2013 Dec 19;4:455.

Notes:

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warning(s):
Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.
Scientific Category:
Immunotherapy
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance