Recombinant HEK293 cell line expressing human ERG potassium channel (ether-a-gogo-related gene, also known as KCNH2 or Kv11.1, accession number NM_000238).
The channel has been validated for activity using a fluorescence-based assay that uses
thallium influx as a surrogate indicator of potassium ion channel activity coupled with a
thallium-sensitive fluorescent dye.
Genbank #
NM_000238
UniProt #
Q12809
Mycoplasma Testing
The cell line has been screened using the PCR-based VenorGeM® Mycoplasma
Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Background
hERG (human ether-a-go-go-related gene) encodes the alpha subunit of a potassium ion channel, Kv11.1. It contains six transmembrane α-helices with a re-entrant “poreloop” between the fifth and the sixth transmembrane helices. This ion channel is best known for its contribution to the electrical activity of the heart that coordinates the heart's beating. When this channel's ability to conduct electrical current across the cell membrane is inhibited or compromised, either by application of drugs or by rare mutations, it can result in a potentially fatal disorder called long QT syndrome. A number of clinically successful drugs in the market exhibit the potential to inhibit hERG, and create a concomitant risk of sudden death as a side-effect, which has made hERG inhibition an important off-target that must be avoided during drug development.
References
1. Beacham, D.W. et al. (2010). J. Biomol. Screen.15(4):441-446
2. Murphy, S.M. et al. (2006) J. Pharmacol. Toxicol. Methods. 54(1):42-55
Storage/Stability
Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Culture Conditions
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS Bioscience, #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1B (BPS Bioscience, #79531): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 μg/ml of Geneticin
Instructions for Use
See data sheet for detailed culturing and assay protocol.
Shipping Temperature
-80°C
Notes
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