Gli Reporter – NIH3T3 Recombinant Cell line (Hedgehog Pathway)

Catalog #: 60409
Size: 2 vials
The hedgehog pathway controls stem cell growth in embryonic and adult tissues and promotes tumor growth in a number of human cancers. The mammalian hedgehog proteins, Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh) activate hedgehog signaling by binding to their membrane receptor “Patched” (PTCH). This binding releases PTCH inhibition of Smoothened (Smo) and allows Smo to activate the Gli family of transcription factors, leading to transcription and expression of hedgehog signal target genes.
The Gli Reporter – NIH3T3 Cell Line is designed for monitoring the activity of the hedgehog signaling pathway. The Gli Reporter – NIH3T3 Cell Line contains the firefly luciferase gene under the control of Gli responsive elements stably integrated into NIH3T3 cells. Luciferase expression correlates with activation of the hedgehog signaling pathway.
Materials Required But Not Provided:

Mouse Sonic Hedgehog (Shh), His-tag (BPS Cat. # 91012)
Cyclopamine (BPS Cat. # 27013): inhibitor of hedgehog pathway (Smo inhibitor)
Vismodegib (GDC-0449) (BPS Cat. #27010): inhibitor of hedgehog pathway (Smo inhibitor)
• Assay medium: Opti-MEM Reduced Serum Medium (Invitrogen #31985-062) with 0.5% calf serum (Hyclone # SH30072.03), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na-pyruvate (Hyclone # SH30239.01), 10 mM HEPES (Hyclone # SH30237.01), and 1% Pen/Strep (hyclone # SV30010)
• Growth Medium 5B (BPS Cat. #79541)
• 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
ONE-Step™ Luciferase Assay System (BPS Cat. #60690)
• Luminometer

Gli cell line, Hedgehog reporter
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
This cell line is validated for its response to stimulation with murine Sonic Hedgehog and to treatment with inhibitors of the hedgehog signaling pathway.
Host Cell line:
NIH3T3 cells (Murine)
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 5 (BPS Cat. #60182): DMEM medium (Hyclone #SH30243.01) supplemented with 10% Calf Serum (CS) (Hyclone # SH30072.03), (not FBS), 1% Penicillin/Streptomycin (Hyclone # SV30010).

Growth Medium 5B (BPS Cat. #79541): Thaw Medium 5 (BPS Cat. #60182) and 500 μg/ml of Geneticin (LifeTechnologies #11811031).

Aqueous solution containing DMSO
Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

• Monitor Hedgehog signaling pathway activity.
• Screen for activators or inhibitors of the Hedgehog signaling pathway.

1. Kinzler KW et al. (1990) Mol Cell Biol. 10(2):634-642.
2. Mullor JL et al. (2002) Trends Cell Biol. 12(12):562-569.
3. Peukert S et al. (2010) ChemMedChem. 5(4):500-512.


License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Cell line
Data shown is lot-specific. Contact us for specific information on other lots.

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