Gli Luciferase Reporter NIH3T3 Cell line (Hedgehog Pathway)

Catalog #
60409
$2,445 *
Size: 2 vials
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Description

The Gli Reporter NIH3T3 Cell Line is designed for monitoring the activity of the hedgehog signaling pathway. This cell line contains the firefly luciferase gene under the control of Gli responsive elements stably integrated into NIH3T3 cells. Luciferase expression correlates with activation of the hedgehog signaling pathway. This cell line has been validated for its response to stimulation with murine Sonic Hedgehog and to treatment with inhibitors of the hedgehog signaling pathway.

Interested in screening and profiling inhibitors or activators of the hedgehog signaling pathway without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 7 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
Gli cell line, Hedgehog reporter
Product Info
Storage and Usage
Citations7
Host Cell Line
NIH3T3 cells (Murine)
Species
Mouse
Supplied As
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Materials Required But Not Supplied

Media Required for Cell Culture 

Name Ordering Information
Thaw Medium 5 BPS Bioscience #60182
Growth Medium 5B BPS Bioscience #79541

 

Materials Used in the Cellular Assay 

Name Ordering Information
Assay Medium 5A BPS Bioscience #79620
Mouse Sonic Hedgehog (Shh) BPS Bioscience #91012
Vismodegib (GDC-0449) BPS Bioscience #27010
Cyclopamine, Free Base BPS Bioscience #27013
ONE-Step™ Luciferase Assay System BPS Bioscience #60690
White clear-bottom 96-well cell culture-treated plate   
Luminometer  
Biological Activity
This cell line is validated for its response to stimulation with murine Sonic Hedgehog and to treatment with inhibitors of the hedgehog signaling pathway.
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The hedgehog pathway controls stem cell growth in embryonic and adult tissues and promotes tumor growth in a number of cancers. The mammalian hedgehog proteins, Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh) activate hedgehog signaling by binding to their membrane receptor “Patched” (PTCH). This binding releases PTCH inhibition of Smoothened (Smo) and allows Smo to activate the Gli family of transcription factors, leading to transcription and expression of hedgehog target genes.

References
  1. Kinzler KW et al. (1990) The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Mol Cell Biol. 10(2): 634-642.
  2. Mullor JL et al. (2002) Pathways and consequences: Hedgehog signaling in human disease. Trends Cell Biol. 12(12): 562-569.
  3. Peukert S et al. (2010) Small-molecule inhibitors of the hedgehog signaling pathway as cancer therapeutics. ChemMedChem. 5(4): 500-512.