Gli Luciferase Reporter NIH3T3 Cell line (Hedgehog Pathway)
The Gli Reporter NIH3T3 Cell Line is designed for monitoring the activity of the hedgehog signaling pathway. This cell line contains the firefly luciferase gene under the control of Gli responsive elements stably integrated into NIH3T3 cells. Luciferase expression correlates with activation of the hedgehog signaling pathway. This cell line has been validated for its response to stimulation with murine Sonic Hedgehog and to treatment with inhibitors of the hedgehog signaling pathway.
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This product has been cited 7 times.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Media Required for Cell Culture
Name | Ordering Information |
Thaw Medium 5 | BPS Bioscience #60182 |
Growth Medium 5B | BPS Bioscience #79541 |
Materials Used in the Cellular Assay
Name | Ordering Information |
Assay Medium 5A | BPS Bioscience #79620 |
Mouse Sonic Hedgehog (Shh) | BPS Bioscience #91012 |
Vismodegib (GDC-0449) | BPS Bioscience #27010 |
Cyclopamine, Free Base | BPS Bioscience #27013 |
ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
White clear-bottom 96-well cell culture-treated plate | |
Luminometer |
The cell line has been screened to confirm the absence of Mycoplasma species.
The hedgehog pathway controls stem cell growth in embryonic and adult tissues and promotes tumor growth in a number of cancers. The mammalian hedgehog proteins, Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh) activate hedgehog signaling by binding to their membrane receptor “Patched” (PTCH). This binding releases PTCH inhibition of Smoothened (Smo) and allows Smo to activate the Gli family of transcription factors, leading to transcription and expression of hedgehog target genes.
- Kinzler KW et al. (1990) The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. Mol Cell Biol. 10(2): 634-642.
- Mullor JL et al. (2002) Pathways and consequences: Hedgehog signaling in human disease. Trends Cell Biol. 12(12): 562-569.
- Peukert S et al. (2010) Small-molecule inhibitors of the hedgehog signaling pathway as cancer therapeutics. ChemMedChem. 5(4): 500-512.