HSP90β, His-tag

Catalog #: 50292
Size: 200 µg
Species: Human
Host Species: E. coli
MW: 83 kDa
Genbank #: AY359878
Tag(s): C-terminal His-tag
a.a: full length
Hsp90 (90 kDa heat shock protein) is a molecular chaperone that aids protein folding and quality control for a large number of client proteins. Functional Hsp90 operates as dimer and has intrinsic ATPase activity. The Hsp90 dimer acts in concert with other chaperones (e.g. Hsp70) and is regulated by a number of co-chaperones/accessory proteins (e.g. Hop, cdc37). Hsp90 has been shown to interact with > 100 proteins and some notable clients include kinases (e.g. Raf-1), nuclear hormone receptors (e.g. estrogen receptors), transcription factors (e.g. p53), GPCRs (e.g. CB2 receptors) and ion channels (e.g. CFTR). In humans, the Hsp90beta isoform is constitutively expressed whereas the Hsp90alpha isoforms is expressed under stress conditions. Hsp90 plays an important role in some tumor cell types by stabilising mutated oncogenic proteins. Inhibition of Hsp90beta function has been shown to play a role in tumorigenesis and disease progression.
Human Heat Shock Protein 90β (GenBank Accession No. AY359878), full length with C-terminal His-tag, MW = 83 kDa, expressed in an E. coli expression system.
HSP90b, Heat Shock Protein 90b, HSP90beta, HSP90β
Assay Conditions:
Assayed using the BPS Hsp90β Assay kit, Cat. # 50294.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl,2 mM DTT, and 20% glycerol.
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
1. Milicevic Z et al., Int J Oncol. 2008 Jun;32(6):1169-78.
2. Chao C.C. et al., J Cell Biochem. 2008 Jul 1;104(4):1286-96.
Avoid freeze/thaw cycles
Scientific Category:
Heat Shock Proteins
Product Type:
Recombinant Protein
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