HSP90ß (N-terminal) Assay Kit

Catalog #

50299

$1,015 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Hsp90β (heat shock protein 90) is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90β function has been shown to play a role in tumorigenesis and disease progression. The Hsp90β Assay Kit is designed for the identification of Hsp90β inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin, an HSP90 inhibitor, for binding to purified recombinant Hsp90β.

The key to the Hsp90β Assay Kit is the fluorescently labeled geldanamycin. Only one simple step on a microtiter plate is required for Hsp90β reactions. The FITC-labeled geldanamycin is incubated with a sample containing Hsp90β enzyme to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

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Synonyms

HSP90b, Heat Shock Protein 90b, HSP90beta, HSP90β

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorescence Polarization

Supplied As

The Hsp90β Assay Kit comes in a convenient 384-well format, with enough purified Hsp90β enzyme, FITC-labeled geldanamycin, and Hsp90β assay buffer for 400 enzyme reactions.

Materials Required But Not Supplied

40 M DTT
2 mg/ml BSA (bovine serum albumin)

Format

Catalog #	Name	Amount	Storage
50292	Hsp90β recombinant enzyme	140 µg	-80°C	Avoid freeze/ thaw cycles!
	FITC-labeled geldanamycin (2.5 µM)	2 x 30 µl	-80°C
50311	5x Hsp90 assay buffer 1	2 x 4 ml	-20°C
79961	Black, low binding NUNC microtiter plate	1	Room Temp

*The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.

UniProt #

P08238

References

1. Kim, J., et al. J Biomol Screening 2004, 9(5): 375-381
2. Howes, R., et al. Anal Biochem 2006, 350: 202-213

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.

Shipping Temperature

-80°C

Warnings

This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note: FP, assay principles and applications.  

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Heat Shock Proteins

Regulatory Status

Research Use Only

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HSP90ß (N-terminal) Assay Kit

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