HSP90α (C-Terminal) Inhibitor Screening Assay Kit

Catalog #

50317

$1,015 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The HSP90α (C-Terminal) Inhibitor Screening Assay Kit is designed to measure the inhibition of HSP90α binding to its protein target PPID (also known as Cyclophilin D). With this kit, only three simple steps on a microtiter plate are required. First, a sample containing HSP90α, PPID, and an inhibitor of choice is incubated for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

Need us to run inhibitor screens or profile your compounds against HSP90α (C-Terminal Domain)? Check out our Heat Shock Protein Screening Services.

This product has been cited 14 times.

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Synonyms

HSP90alpha, Heat Shock Protein 90 alpha, activity assay kit, HSP90a

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Product Info

Storage and Usage

Citations14

Assay Kit Format

AlphaLISA®

Supplied As

The assay kit comes in a convenient AlphaLISA® format, with enough HSP90α (535-732), assay buffer, detection buffer, and purified GST-tagged PPID to perform a total of 384 enzyme reactions.

Format

Catalog #	Component	Amount	Storage
50316	HSP90α (C-terminal), Biotin-labeled	5 µg	-80°C	Avoid freeze/ thaw cycles!
71095	PPID, GST-tag	2 x 10 µg	-80°C
50324	3x HSP90 Assay Buffer 2	4 ml	-20°C
	3x HSP90 Detection Buffer	3 ml	-20°C

*The concentration of HSP90 and PPID are lot-specific and will be indicated on the tubes containing the protein

UniProt #

P07900

References

Allan, R.K. et al. J. Biol.Chem 2006. 281(11): 7161-71.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Applications

Useful for screening inhibitors of HSP90α and for HSP90α binding assays

Shipping Temperature

-80°C

Notes

AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.

Contraindications

Only limited amounts of DMSO (< 1%) can be included as it has been shown to disrupt HSP90α:PPID interaction. Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Heat Shock Proteins

Regulatory Status

Research Use Only

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HSP90α (C-Terminal) Inhibitor Screening Assay Kit

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