HDAC9, His-tag Recombinant

Catalog #

50009

$475 *

Size: 10 µg

Qty

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Description

Human HDAC9 , GenBank Accession No. NM_178423, a.a. 604-1066 with C-terminal His-tag, MW= 50.7 kDa, expressed in baculovirus expression system.

This product has been cited 23 times.

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Synonyms

histone deacetylase 9, HDAC-9

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

HDAC9 inhibition curves using a fluorogenic assay (#50069). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations23

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥77%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 200 mM imidazole

50.7 kDa

Amino Acids

604–1066

Specific Activity

≥3000 pmol/min/µg

Genbank #

NM_178423

UniProt #

Q9UKV0

Tag(s)

C-terminal His-tag

References

1. Han et al., (2005) J.Mol. Biol. 345, 91-102
2. Lomonte et al., (2004) J. Virol 78, 6744-6757.

Storage/Stability

>6 months at -80°C.

Assay Conditions

25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl₂, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC9 (0.15 - 10 ng). Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only