HDAC6 (H611A), GST-tag Recombinant

Catalog #

50066

$465 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human HDAC6 with H611A mutation (GenBank Accession No. NM_006044), full length with Nterminal GST tag, MW= 161 kDa, expressed in a baculovirus expression system.

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Synonyms

histone deacetylase 6, HDAC6, HDAC-6

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥48%

Purification

Protein was purified by affinity chromatography and gel filtration.

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% Glycerol.

161 kDa

Amino Acids

full length

Specific Activity

0.83 pmole/min/µg

Genbank #

NM_006044

UniProt #

Q9UBN7

Tag(s)

N-terminal GST-tag

References

1. Li S. et al., Neurology. 2010; 41(2): 112-6.

2. Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 2002; 99(26): 16899-16903.

Application References:
1. Kinetic method for the large-scale analysis of the binding mechanism of histone deacetylase inhibitors (2014)
2. Potent histone deacetylase inhibitors derived from 4-(aminomethyl)-N-hydroxybenzamide with high selectivity for the HDAC6 isoform (2013)
3. Santacruzamate A, a potent and selective histone deacetylase inhibitor from the Panamanian marine cyanobacterium cf. Symploca sp (2013)
4. Thioester derivatives of the natural product psammaplin A as potent histone deacetylase inhibitors (2013)

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC6 H611A (15 – 1000 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Deacetylase

Regulatory Status

Research Use Only