HDAC6, GST-Tag (Mouse) Recombinant

Catalog #

50057

$440 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Mouse HDAC6, also known as histone deacetylase 6, GenBank Accession No. NM_010413, a.a. 2-1149 (end) with N-terminal GST-Tag, expressed in baculovirus expression system. MW = 152 kDa.

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Synonyms

histone deacetylase 6, HDAC 6, HDAC-6

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 cells

Purity

≥77%

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol

152 kDa

Amino Acids

2-1149 (end)

Genbank #

NM_010413

Tag(s)

N-terminal GST tag

References

1. Rikimaru, T. et al., Oncology 72(1-2), 69-74 (2007).

2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Assay Conditions

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and Mouse HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Scientific Category

HDAC

Regulatory Status

Research Use Only