Recombinant human HDAC6 (histone deacetylase 6), full length encompassing amino acids 1-1215(end). This protein contains a C-terminal FLAG-Tag and was affinity purified.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol
MW
132 kDa
Amino Acids
1-1215(end)
Specific Activity
≥350 pmol/min/µg.
Genbank #
NM_006044
UniProt #
Q9UBN7
Tag(s)
C-terminal FLAG-tag
Background
HDAC6 is a distinct member of the histone deacetylase family, primarily found in the cytoplasm. Unlike other HDACs it targets non-histone proteins. HDAC6 regulates cell motility by deacetylating α-tubulin and helps degrade misfolded proteins through autophagy. Additionally, HDAC6 influences stress responses and immune signaling by modulating acetylation of key regulatory proteins. Due to its versatile roles, HDAC6 is a promising drug target with potential therapeutic applications in cancer, neurodegenerative diseases, and immune disorders.
References
Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).
Storage/Stability
At least 6 months at –80°C
Assay Conditions
Assay run according to HDAC6 Fluorogenic Assay Kit (BPS Bioscience #50076) with HDAC6 titrated at various concentrations.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.