HDAC5, GST-Tag, His-Tag Recombinant

Catalog #

100102

$465 *

Size: 5 µg

Qty

Bulk/Custom

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Description

Human HDAC5 (GenBank Accession No. NM_005474), a.a. 1-1122 (end) with N-terminal GST-tag and C-terminal His-tag, expressed in a HEK293 expression system. MW=149 kDa.

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Synonyms

histone deacetylase 5, HDAC-5

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Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥55%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16mM Glutathione, 20% glycerol.

149 kDa

Amino Acids

1-1122

Specific Activity

≥1000 pmol/min/µg

Genbank #

NM_005474

UniProt #

Q9UQL6

Tag(s)

N-terminal GST-tag, C-terminal His-tag

References

1. Spiegelberg, B.D. and Hamm, H.E. (2005) J. Biol. Chem. 280, 41769-41776

2. Waltregny et al., (2004) J. Neural Transm. 48, 273-290.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 1 (Catalog #50040), and HDAC5 (0.3125 – 20 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

HDAC

Regulatory Status

Research Use Only