HDAC1, FLAG-Tag, His-Tag Recombinant

Catalog #

50051

$485 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Human recombinant HDAC1 (Histone deacetylase 1), full length, encompassing amino acids 1-482(end). This construct contains a C-terminal His-tag (6xHis) followed by a FLAG-tag. This recombinant protein was affinity purified as a complex with endogenous tubulin.

This product has been cited 177 times.

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Synonyms

Histone deacetylase 1, HD1, Protein deacetylase HDAC1, Protein decrotonylase HDAC1, RPD3L1, HDAC-1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

HDAC1 Activity

HDAC1 inhibition curves using a fluorogenic assay (#50061). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations177

Species

Human

Construct

HDAC1/Tubulin (1-482(end)-His-FLAG)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 100 ng/µl FLAG peptide, and 20% Glycerol

56 kDa + Tubulin (55 kDa)

Amino Acids

1-482(end)

Specific Activity

460 pmol/min/µg.

Genbank #

NM_004964

UniProt #

Q13547

Tag(s)

C-terminal His-tag, C-terminal FLAG-tag

Background

HDAC1, or histone deacetylate 1, is a Class I member of the histone deacetylase family which is involved in lysine deacetylation. Lysine acetylation/deacetylation is a dynamic process involved in the regulation of a variety of cellular functions, similarly to phosphorylation/dephosphorylation. HDAC1 is part of the histone deacetylase complex, localized in the nucleus, and regulates eukaryotic gene expression. It can also directly interact with different transcription factors to regulate specific pathways, for example: HDAC1 interacts with MTA-2 (metastasis-associated protein-2) to deacetylate p53 and thus participates in cell growth and apoptosis regulation. In endothelial cells HDAC regulates angiogenesis, inflammation, redox and nitric oxide signaling, in response to environmental stimuli. Dysfunction of HDAC1 can contribute to atherosclerosis. Mutations in HDAC1 can result in cancer, via the deregulation of genes involved in cell proliferation and survival. A number of HDAC inhibitors have been approved for the treatment of cancer, but most are non-selective. Entinostat (or MS-275) is an inhibitor that acts preferentially on HDAC1 and has shown promising results in phase I clinical trials for refractory solid tumors and lymphoma. The development of new inhibitors specifically targeting HDAC1 may open newer avenues for cancer and HDAC1-linked diseases in the endothelium.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to HDAC1 Fluorogenic Assay Kit (BPS Bioscience #50061) with HDAC1 titrated at various concentrations

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Notes

Recombinant HDAC1 forms a complex with endogenous Hsp70. The identity of Hsp70 was confirmed by MALDI-TOF mass spectrometry.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Deacetylase

Regulatory Status

Research Use Only

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Datasheets/Protocols

50051 Lot 220719

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HDAC1, FLAG-Tag, His-Tag Recombinant

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