HDAC developer (2X)

Catalog #
50030
$230 *
Size: 6 ml
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Description

2x HDAC Assay Developer is a reagent designed to be used with BPS Bioscience HDAC (histone deacetylase) or sirtuin fluorogenic assay kits containing Fluorogenic HDAC/SIRT Substrate 1 (BPS Bioscience #50032), Fluorogenic HDAC Class 2a Substrate (BPS Bioscience #50040), Fluorogenic HDAC Class 2a Substrate 2 (Green) (BPS Bioscience #50042), Fluorogenic HDAC Substrate 2 (Green) (BPS Bioscience #50038) and Fluorogenic HDAC Substrate 3 (BPS Bioscience #50037). Use of an appropriate fluorogenic substrate, containing acetylated lysine side chain, and incubation with a sample containing HDAC activity (purified enzyme) results in deacetylation of the substrate. This deacetylation of the substrate allows the generation of a fluorescent signal when incubated with HDAC Assay Developer. Trichostatin A is included in the formulation to stop Class 1 and 2 HDACs reactions and allow measurement of HDAC activity at the time of addition.

This product has been cited 31 times.

Product Info
Storage and Usage
Citations31
Supplied As
2x Concentrate
Format
Aqueous buffer solution
References

1. K. Ito et al. Proc. Natl. Acad. Sci. USA 2002 99 8921.
2. B. Heltweg and M. Jung Anal. Biochem. 2002 302 175.
3. S. Milutinovic et al. J. Biol. Chem. 2002 277 20974