HDAC Assay Buffer

Catalog #

50031

$45 *

Size: 20 ml

Qty

Bulk/Custom

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Description

HDAC assay buffer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.) in HDAC assay buffer. Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included as an ‘inhibitor stop’ for class I and II HDACs.

This product has been cited 67 times.

Product Info

Storage and Usage

Citations67

Supplied As

Tris-buffered solution

Format

Aqueous buffer solution

Storage/Stability

Stable at least 12 months from date of receipt, when stored as directed

Instructions for Use

In a 50 µL reaction, assay HDAC activity using HDAC (enzyme, lysate, etc.), substrate, HDAC assay buffer, and test inhibitor. Incubate microtiter plate at 37°C for 30 minutes. Add an equal volume (50 µL) of HDAC Assay Developer to each well. Incubate the plate at room temperature for 15 minutes. Read sample in a microtiter-plate reading fluorimeter capable of excitation at a wavelength in the range of 350-380 nm and detection of emitted light in the range of 440-460 nm. “Blank” value (no enzyme negative control) is subtracted from all other values.

Applications

Suitable for use with cell extracts, nuclear extracts, immunoprecipitates, and purified HDAC enzymes.

Shipping Temperature

-20°C

Warnings

Do not dilute! Avoid freeze/thaw cycles

Scientific Category

Deacetylase

Regulatory Status

Research Use Only

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HDAC Assay Buffer

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