HCV1b (R155Q), FLAG-His-tags Recombinant

Catalog #
80104
$415 *
Size: 20 µg
Qty
*US Pricing only. For international pricing, please contact your local distributor.
Purchase
Description

Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1b (GenBank Accession No. AF054247) with Arg-to-Gln mutation on a.a. 155, an N-terminal FLAG-His tag, and a 4 a.a. linker, MW = 22.3 kDa, expressed in an E. coli expression system.

 

Synonyms
hepatitis c virus genotype 1b Q155
Product Info
Storage and Usage
Citations
Species
Hepatitis C Virus
Host Species/Expression System
E. coli
Purity

≥80%

Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 335 mM imidazole, and 20% glycerol.
MW
22.3 kDa
Amino Acids
21–32 of NS4A, linker (4 a.a.) and a.a. 3–181 of NS3
Amino Acid Sequence
MDYKDDDDKHHHHHHGSVVIVGRIILSGSGSITAYSQQTRGVLGCIITSLTGRDKNQVEGEVQVVSTATQSFLATCINGVCWTVYHGAGSKTLAGPKGPITQMYTNVDLDLVGWQAPPGARSMTPCSCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPVSYLKGSSGGPLLCPSGHVVGVFQAAVCTRGVAKAVDFIPVESMETTMRS
Genbank #
AF054247
UniProt #
O92972
Tag(s)
N-terminal FLAG-His-tags
References
1. Lin, C., et al., Hepatitis C Viruse. Norfolk (UK): Horizon Bioscience; 2006. Chapter 6.
2. Lang Kuhs, K.A., et al., Mol. Ther. 2012 May;20(3):669-678.
3. Massariol, MJ., et al., Biochem Biophys Res Commun. 2010 Jan 1;391(1):692-697.