GSK3β

Catalog #: 40007
Size: 10 µg
$355
Species: Human
Host Species: Sf9 insect cells
MW: 73 kDa
Genbank #: NM_002093
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human GSK3β GenBank Accession No. NM_002093), full length, with N-terminal GST-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
P49841
Synonym(s):
GSK3b, glycogen synthase kinase 3 beta, GSK3beta
Specific Activity:
391 pmol/min/µg
Assay Conditions:
Enzyme reactions include 50 μL volumes of 1x Kinase buffer (40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA) along with 20 μM ATP, 0.2 mg/mL GSKtide substrate, 3mM DTT, and GSTGSK3 . After 45 min incubation at 30°C, 50μL of the Kinase-Glo reagent solution was added and luminescence from remaining ATP was measured.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

40007_Lot150501-G2.pdf