GSK3β, GST-tag, His-tag Recombinant

Catalog #

40007

$365 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human GSK3β (glycogen synthase kinase 3 beta), full length encompassing amino acids 2-433 (end). The construct contains an N-terminal GST-tag and a C-terminal His-tag (6xHis). The protein was affinity purified.

This product has been cited 12 times.

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Synonyms

GSK3b, glycogen synthase kinase 3 beta, GSK3beta, Serine/threonine-protein kinase GSK3B

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations12

Species

Human

Construct

GSK3ß (GST-2-433(end)-His)

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Supplied As

Aqueous buffer solution.

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 250 mM Imidazole and 20% glycerol.

75 kDa

Amino Acids

2-433(end)

Specific Activity

≥300 pmol/min/µg

Genbank #

NM_002093

UniProt #

P49841

Tag(s)

N-terminal GST-tag, C-terminal His-tag

References

1. Sutherland C. et al. Biochem J. 1993 Nov 15;296 (Pt 1):15-9.
2. Sperber BR et al. Neurosci Lett. 1995 Sep 8;197(2):149-53.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to the GSK3β Assay Kit (BPS Bioscience #79700) with GSK3β titrated from 48 ng/rxn – 0.75 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40007 Lot 210720-2

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GSK3β, GST-tag, His-tag Recombinant

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